《植物生理学报》 2012, 48(12): 1173-1178

橡胶树未成苗体细胞胚的茎芽分化及利用

李玲*, 梁国平, 管艳, 黄凤翔, 肖三元, 田海

云南省热带作物科学研究所, 云南景洪666100

通信作者：李玲；E-mail: liling2612@163.com；Tel: 0691-2120332

摘　要：

以橡胶树未成苗体细胞胚为试材, 选取未成苗双子叶胚、连体胚、多子叶胚、单子叶胚和子叶愈伤化胚作为外植 体, 研究不同体细胞胚类型的外植体分化茎芽的情况, 并利用其进行幼态微型芽条的培育研究。结果表明: 在植株诱导培 养基中培养80d后, 未成苗双子叶胚、连体胚、多子叶胚、单子叶胚、子叶愈伤化胚均能分化出芽, 双子叶胚分化率最高 (达90%), 单子叶胚最低(20%), 多子叶胚和连体胚最多可分化出芽3个, 双子叶胚2个。幼态微型芽条增殖的最优培养基为 MS+6-BA 2 mg•L-1或MS+6-BA 2 mg•L-1+KT 1 mg•L-1, 而添加NAA对芽的伸长有抑制作用; 适宜幼态微型芽条伸长培养的基 本培养基为MS。

关键词：橡胶树; 体细胞胚类型; 未成苗体细胞胚; 幼态微型芽条

收稿：2012-09-24   修定：2012-12-04

资助：国家科技支撑计划项目(2011BAD30B01)、农业部国 家科技支撑计划(2006BAD01A06-3)、科技创新强省计划(2009AB001)、云南省产业重大关键技术开发专项计划([2008]1657号)、云南农垦重点科技计划项目 (2006XJ03)。

Bud Differentiation and Utilization of Non-Seedling Somatic Embryos of Hevea brasiliensis Muell. Arg.

LI Ling*, LIANG Guo-Ping, GUAN Yan, HUANG Feng-Xiang, XIAO San-Yuan, TIAN Hai

Yunnan Tropical Crops Research Institute, Jinghong, Yunnan 666100, China

Corresponding author: LI Ling; E-mail: liling2612@163.com; Tel: 0691-2120332

Abstract:

Non-seedling somatic embryos of Hevea brasiliensis, including dicotyledonous embryo, connection radicle embryo, polycotyledonous embryo, monocotyledonous embryo and cotyledon recallus embryo, were used as explants in this study. The bud differentiation and the mini-juvenile-type bud stick cultivation of all these somatic embryos were studied. The results indicate that all 5 types of embryos can develop buds after they were cultured in an induction medium for 80 d. The highest differentiation rate was 90% found in dicotyledonous embryos, and the lowest differentiation rate was 20% found in monocotyledonous embryos. Polycotyledonous embryo and connection radicle embryo could differentiate 3 buds at most, 2 for dicotyledonous embryo. The optimal medium for the propagation of mini-juvenile-type bud stick was MS+6-BA 2 mg·L^-1 or MS+6-BA 2 mg·L^-1+KT 1 mg·L^-1. The optimal basic medium for elongation of mini-juvenile-type bud stick was MS. With addition of NAA, the elongation of shoots can be inhibited.

Key words: Hevea brasiliensis; classification of somatic embryos; non-seedling somatic embryos; mini-juvenile-type bud stick